Premium N-terminal lysine proteases purified from mushrooms for advanced proteomics applications.
Unique specificity for lysine residues on the N-terminal side, opposite to trypsin. Provides complementary peptide coverage for comprehensive protein analysis.
Purified from mushrooms using proprietary methods. Every batch is tested for activity to ensure consistent performance.
Rigorous quality control ensures reproducible digestion efficiency across batches. Reliable performance for your critical experiments.
Optimized storage buffer maintains activity during shipping and storage. Stable at -20°C for extended periods.
Generates peptides ideal for mass spectrometry. Enhanced ion mobility separation improves DIA proteomics performance.
Suitable for sensitive LC-MS/MS quantification of Aβ40 and Aβ42 in CSF and blood biomarker assays.
| Source | Purified from mushrooms (G. frondosa) |
|---|---|
| Origin | Made in the USA |
| Specificity | N-terminal side of lysine residues |
| Cleavage Site | ↓K (opposite to trypsin K↓) |
| Activity | Tested and certified for each batch |
| Storage Temperature | -20°C |
| Stability | 12 months at -20°C |
| Optimal pH | pH 7.5 - 8.5 |
| Optimal Temperature | 37°C |
| Recommended Ratio | 1:50 - 1:100 (enzyme:substrate, w/w) |
| Digestion Time | 4-16 hours (overnight recommended) |
N- and C-terminal Measurement: Lys-N digestion overcomes the challenges of measuring the N- and C-terminal regions of Aβ and is essential for sensitive LC-MS/MS quantification of Aβ40 and Aβ42 in CSF and blood biomarker assays.
Clinical Utility: Supports accurate biomarker quantification in diagnostic applications.
Improved Ion Mobility Separation: LysN-generated peptides exhibit greater spread in the ion mobility dimension compared to tryptic peptides.
Enhanced Coverage: Better peak separation leads to improved peptide identification and quantification in complex samples.
Complementary to Trypsin: N-terminal cleavage generates different peptide sets, filling gaps in sequence coverage.
PTM Mapping: Alternative cleavage strategy helps identify and localize post-translational modifications missed by trypsin digestion.
Shotgun Proteomics: Reliable enzyme for discovery proteomics in complex biological samples.
Sample Compatibility: Performs consistently across plasma, serum, tissue, cell lysates, and other sample types.
Label-Free Quantification: Compatible with intensity-based and spectral counting methods.
Labeled Quantification: Works with TMT, iTRAQ, SILAC, and other multiplexing approaches.
Alternative Strategy: Useful for proteins with limited lysine accessibility or unusual amino acid distributions.
Membrane Proteins: Different cleavage pattern can improve coverage of hydrophobic regions.
See how LysN proteases improve DIA proteomics, biomarker quantification, and comprehensive protein analysis.
Science & Applications